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lentiviral vectors pseudotyped with vesicular stomatitis virus glycoprotein (vsv-g) and packaging plasmid ppax2  (Addgene inc)


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    Structured Review

    Addgene inc lentiviral vectors pseudotyped with vesicular stomatitis virus glycoprotein (vsv-g) and packaging plasmid ppax2
    Lentiviral Vectors Pseudotyped With Vesicular Stomatitis Virus Glycoprotein (Vsv G) And Packaging Plasmid Ppax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/packaging+vectors+ppax2/pax2+plasmid/pm40346583-43-10-16
    Average 90 stars, based on 1 article reviews
    lentiviral vectors pseudotyped with vesicular stomatitis virus glycoprotein (vsv-g) and packaging plasmid ppax2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    shRNA:

    Article Title: eIF4A supports an oncogenic translation program in pancreatic ductal adenocarcinoma
    Article Snippet: Isotope labelling: Organoids were incubated for 30 min in media containing 10 μCi of [ 35 S] labelled Methionine (Perkin Elmer Cat# NEG709A005MC). .. pLenti-Cas9, LRC2.1T-sgRNA and human pPrime-PGK-Puro shRNA lentiviruses were produced in 293 T cells co-expressing the packaging vectors (pPAX2, Addgene# 12260 and VSVG, Addgene# 12259), concentrated with LentiX concentrator (Clontech Cat# 631232), and resuspended with DMEM supplemented with 10%FBS and 1% PS at 5× concentration. .. Mouse MLS shRNA retroviruses were produced in Phoenix-ECO cells (ATCC Cat# CRL-3214, RRID:CVCL_H717), concentrated with RetroX Concentrator (Clontech Cat# 631456), and resuspended in DMEM supplemented with 10% FBS and 1% PS, or Advanced DMEM/F12 (Gibco Cat#12634010) supplemented with 1% Penicillin/Streptomycin (PS), 1× GlutaMAX (Gibco Cat# 35050061), and 1× Hepes (Gibco Cat# 15630080) at 5× concentration.

    Produced:

    Article Title: eIF4A supports an oncogenic translation program in pancreatic ductal adenocarcinoma
    Article Snippet: Isotope labelling: Organoids were incubated for 30 min in media containing 10 μCi of [ 35 S] labelled Methionine (Perkin Elmer Cat# NEG709A005MC). .. pLenti-Cas9, LRC2.1T-sgRNA and human pPrime-PGK-Puro shRNA lentiviruses were produced in 293 T cells co-expressing the packaging vectors (pPAX2, Addgene# 12260 and VSVG, Addgene# 12259), concentrated with LentiX concentrator (Clontech Cat# 631232), and resuspended with DMEM supplemented with 10%FBS and 1% PS at 5× concentration. .. Mouse MLS shRNA retroviruses were produced in Phoenix-ECO cells (ATCC Cat# CRL-3214, RRID:CVCL_H717), concentrated with RetroX Concentrator (Clontech Cat# 631456), and resuspended in DMEM supplemented with 10% FBS and 1% PS, or Advanced DMEM/F12 (Gibco Cat#12634010) supplemented with 1% Penicillin/Streptomycin (PS), 1× GlutaMAX (Gibco Cat# 35050061), and 1× Hepes (Gibco Cat# 15630080) at 5× concentration.

    Article Title: A Multiprotein Supercomplex Controlling Oncogenic Signaling in Lymphoma
    Article Snippet: Colonies were scrapped, spun and DNA was isolated with Blood and Cell Culture DNA Maxi kits (Qiagen). .. Lentiviruses were produced in 293FT cells by transfecting sgRNA vectors with packaging vectors pPAX2 (Addgene #12260) and pMD2.g (Addgene #12259) in a 4:3:1 ratio in serum-free Opti-MEM. .. Trans-IT 293T (Mirus) was added and incubated for 15 minutes before adding dropwise to cells.

    Concentration Assay:

    Article Title: eIF4A supports an oncogenic translation program in pancreatic ductal adenocarcinoma
    Article Snippet: Isotope labelling: Organoids were incubated for 30 min in media containing 10 μCi of [ 35 S] labelled Methionine (Perkin Elmer Cat# NEG709A005MC). .. pLenti-Cas9, LRC2.1T-sgRNA and human pPrime-PGK-Puro shRNA lentiviruses were produced in 293 T cells co-expressing the packaging vectors (pPAX2, Addgene# 12260 and VSVG, Addgene# 12259), concentrated with LentiX concentrator (Clontech Cat# 631232), and resuspended with DMEM supplemented with 10%FBS and 1% PS at 5× concentration. .. Mouse MLS shRNA retroviruses were produced in Phoenix-ECO cells (ATCC Cat# CRL-3214, RRID:CVCL_H717), concentrated with RetroX Concentrator (Clontech Cat# 631456), and resuspended in DMEM supplemented with 10% FBS and 1% PS, or Advanced DMEM/F12 (Gibco Cat#12634010) supplemented with 1% Penicillin/Streptomycin (PS), 1× GlutaMAX (Gibco Cat# 35050061), and 1× Hepes (Gibco Cat# 15630080) at 5× concentration.



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